Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry

[Selecting reagents for multicolor BD flow cytometry].

Reagent selection starts with your instrument configuration. The type and number of lasers and detectors dictate whether the optical system can excite a given fluorochrome and properly detect a given combination of fluorochromes. The design of the optical system also impacts the efficiency with which particular dyes are detected, as do the instrument settings, including PMT voltages. (See the B...

Minimal residual disease detection in cryopreserved ovarian tissue by multicolor flow cytometry in acute myeloid leukemia.

Multicolor flow cytometry analyses of cellular immune response in rhesus macaques.

The rhesus macaque model is currently the best available model for HIV-AIDS with respect to understanding the pathogenesis as well as for the development of vaccines and therapeutics(1,2,3). Here, we describe a method for the detailed phenotypic and functional analyses of cellular immune responses, specifically intracellular cytokine production by CD4+ and CD8+ T cells as well as the individual...

Detection of bacterial aggregation by flow cytometry

Since the advent of recombinant DNA technology, bacteria have been used often to express foreign proteins [1]. Soluble proteins expressed by bacteria can be expressed either in the cytoplasm or they are secreted to the periplasm or outside the cell. In general proteins expressed in the cytoplasm are expressed to higher levels than if they are secreted[1]. Many hydrophobic and highly expressed p...

Detection of intracytoplasmic cytokines by flow cytometry.

Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years t...